FastQCFastQC Report
Fri 10 Feb 2017
ERR1148381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874442
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229260.7975808870034601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128430.44679976148414197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98460.3425360469962518No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA79350.27605357839886835No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT77230.2686782338972225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.17909562969091045No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39770.13835728812757397No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31460.10944732925555639No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA31130.10829928034728131No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA28920.10061083159792406No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC28920.10061083159792406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2600.043.461442
ATGGGGG57700.031.2318885
CATGGGG94150.028.8055364
GTATCAA377450.027.2669981
AACGCAG386750.026.9423316
TGGGGGG44700.026.4775036
GTAAGTA20950.024.6978452
TATCAAC423850.024.2749212
CATGGGT37600.024.2010964
ATCAACG423950.024.1994723
TCAACGC430300.024.1049694
CAACGCA433450.023.9983985
ACGCAGA434300.023.9788157
CGCAGAG437200.023.8741768
GGGTTAG7500.023.810131
ATCACGC5600.023.3650153
GCAGAGT452450.023.0037639
GAGTACT171000.022.73021712-13
TCACGCA5250.022.6569844
TGTAAGT23050.022.467261
Produced by FastQC (version 0.11.5)