FastQCFastQC Report
Fri 10 Feb 2017
ERR1148380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3838422
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359870.9375467314432858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195100.5082817887142165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158330.4124872147981644No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA147560.38442880954725667No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT137090.3571519754732544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80060.208575294743517No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG65750.17129434960512419No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG55800.1453722389044248No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC54090.1409172831960634No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA53430.13919782660687124No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA50130.13060054366091065No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47790.12450428848104769No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC46310.12064853734164718No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT43990.11460438690691123No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG43080.11223362100363117No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC40860.1064499942945304No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39420.10269845264538396No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39220.10217740519411363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG90300.034.0493625
CATGGGG140600.031.9350664
TGGGGGG75250.030.5851546
GGGTTAG15700.029.5715891
CATGGGT57750.027.9076634
GTATCAA618700.025.2154451
AACGCAG623450.025.1258246
GGTTAGA18850.024.924242
TATCACG2901.4733814E-1024.608742
GTTAGAG19350.024.5875453
TAGAGCA20500.023.4983485
ATGGGTG35550.022.9185495
GAGTACT244000.022.91220312-13
ACGCAGA686950.022.777287
TATAACG4700.022.7761762
TATCAAC687300.022.7483772
AGTACTT251700.022.67203712-13
CGCAGAG688950.022.6688
ACATGGG570950.022.603093
ATCAACG688550.022.5688863
Produced by FastQC (version 0.11.5)