FastQCFastQC Report
Fri 10 Feb 2017
ERR1148377.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148377.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960538
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246480.8325513808638835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143740.48551986159272403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114480.3866864738773831No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88540.299067264125642No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT88450.29876326532542397No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53580.18098061906315677No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37750.1275106078692454No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37170.12555150449006228No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG34830.1176475356843925No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33270.11237822314727933No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA33250.11231066785834197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG13850.039.1097761
ACGCATA2400.037.1731077
ATGGGGG74650.033.941315
GTTAGAG16200.033.7770463
GGTTAGA15850.033.77072
TTAGAGC16950.032.633384
CATGGGG114650.032.4749954
GTACATT15050.032.43181
TGGGGGG62900.029.9748826
TAGAGCA18300.029.9009915
TATCACG2800.029.7369792
GCGTATC2252.055458E-1029.100731
GTATCAA402050.028.3519151
AACGCAG406650.027.9650736
CATGGGT36300.027.3626824
TACAACG3800.026.6067712
AACGCAT3600.026.434216
TAGATCG1150.001007461125.8595525
TATCAAC451000.025.3324762
ATCAACG448700.025.3310663
Produced by FastQC (version 0.11.5)