FastQCFastQC Report
Fri 10 Feb 2017
ERR1148376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704589
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345540.932735048341395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193380.522001226046938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154900.41813005437310324No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA120210.3244894372898046No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT112470.30359643134501557No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77410.20895705299562245No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG54940.1483025512411768No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC53570.1446044352018537No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG47120.1271935969145295No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA44170.11923050033350528No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC43410.11717899070585158No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA43240.11672010039440273No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT37840.10214358461896854No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC37520.1012797910915354No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37260.10057795885049596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG23150.040.3645631
GTTAGAG28500.034.2233283
ATGGGGG98950.032.8771935
GGTTAGA28450.032.8201522
CATGGGG143500.031.4152534
TTAGAGC31950.031.0862874
TAGAGCA31800.030.671855
TGGGGGG83550.029.1850646
CATGGGT46250.029.061634
TATCACG3000.027.7542822
AACGCAT3900.027.449296
GTATCAA565350.026.8246251
AACGCAG573850.026.4073166
ACGCATA3500.025.4886267
GTAAGTA36200.025.3008672
TGTAAGT36200.024.826761
TATAACG3700.024.1108652
TATCAAC630100.023.9743842
ATCAACG629950.023.9140073
ATGGGTG32600.023.8988485
Produced by FastQC (version 0.11.5)