FastQCFastQC Report
Fri 10 Feb 2017
ERR1148374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3251066
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298510.9181911409980602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160710.4943301673974014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131970.40592839394832336No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA129500.3983308859309531No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT120070.36932501524115474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68360.21026949314471008No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG65870.20261046684379833No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50830.15634871762062041No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA48960.15059675810949394No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG48220.1483205816184599No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41540.1277734749156123No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC41340.12715829208019772No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA38260.11768447641481287No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT37140.11423945253649109No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC36640.11270149544795462No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC35990.11070215123285716No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33020.10156668612695036No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC32950.10135137213455525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3150.043.427352
ATGGGGG62900.031.7727435
CGCATAG3600.029.7630081
TATAACG2401.2732926E-1129.7382932
CATGGGG104500.029.7112054
GGGTTAG8350.027.0896871
TGGGGGG54750.026.8335956
ATCACGC5250.026.057613
CATGGGT52700.025.5072654
AACGCAG544050.024.6860246
GTATCAA550000.024.5247211
ACGCAGA598050.022.387427
AGTACTT225400.022.36343612-13
GTAAGTA25300.022.3331052
CGCAGAG600600.022.3121768
GAGTACT216600.022.0912512-13
ATCAACG604600.022.076023
TCAACGC612700.022.0171574
CAACGCA616000.021.8702415
TATCAAC612900.021.8633942
Produced by FastQC (version 0.11.5)