FastQCFastQC Report
Fri 10 Feb 2017
ERR1148372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554605
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264570.7443021095170912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147800.4157986611733231No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA126420.35565133116056497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121200.3409661551705464No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT116490.3277157377542652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63580.17886656885926847No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG59780.1681762108588718No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC52610.14800519326338651No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50270.14142218333682646No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA50050.1408032678736456No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT44710.1257805016309829No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC41840.11770646808857806No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG38600.10859153126718721No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC37400.10521562874074616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3200.037.1719632
ATGGGGG63900.033.135755
CATGGGG105600.031.0338274
GGGTTAG14800.030.1640361
TGGGGGG53600.029.0726186
GGTTAGA16000.026.7638132
CATGGGT46400.024.9956444
GTTAGAG18400.024.566513
AACGCAG513850.024.0523136
GTATCAA518200.023.869211
ACGCATA2802.6248017E-923.3658897
TAGAGCA19600.023.0624375
TACAACG4700.022.7777122
TTAGAGC19650.022.3983944
GTACATT15900.022.087411
ACGCAGA563700.021.8619757
AGTACTT207200.021.71565812-13
GAGTACT201200.021.71288912-13
CGCAGAG567500.021.6736628
TCAACGC572250.021.6704484
Produced by FastQC (version 0.11.5)