FastQCFastQC Report
Fri 10 Feb 2017
ERR1148371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301547
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212410.6433650649225954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124930.37839836900701396No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA98080.2970728570576157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97710.29595217030077114No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT92780.28101977648659854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48460.1467796763153758No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG46080.1395709344740511No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC38900.11782355362501276No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA37970.11500669231726823No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37850.1146432263420754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3550.033.5072362
GGGTTAG11350.029.3677921
ATGGGGG58750.029.1564585
CATGGGG96950.028.34294
GGTTAGA12300.025.6275882
GTATCAA408200.024.4533961
AACGCAG411650.024.4179236
TGGGGGG46500.024.302496
GTAAGTA20500.024.0802612
TATAACG3154.1472958E-1022.6572722
GAGTACT162300.022.46425812-13
GTTAGAG15700.022.3512843
ACGCAGA453400.022.0776447
ATATGTA15400.022.0309521
TCAACGC456050.021.9623994
ATCAACG452550.021.921973
TATCAAC454850.021.9150662
CAACGCA458250.021.8958955
CGCAGAG456600.021.8838378
AGTACTT170300.021.74076512-13
Produced by FastQC (version 0.11.5)