FastQCFastQC Report
Fri 10 Feb 2017
ERR1148370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003003
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244880.8154504008154505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136520.45461160045461163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109720.36536760036536764No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA84050.2798865002798865No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT78980.2630034002630034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58760.1956708001956708No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG43460.1447218001447218No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32500.108225000108225No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32310.10759230010759231No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32230.10732590010732589No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC30840.1026972001026972No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA30410.10126530010126529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG67600.033.1689725
CATGGGG103500.032.7545664
TGGGGGG56250.030.6628746
GGGTTAG9900.027.6552961
ATATGTA16400.027.5819761
TATCACG2706.002665E-1126.433952
CATGGGT38000.025.9813584
GTACATT12700.025.7759481
ACGCATA2558.8766683E-1025.6560557
GTATCAA421550.025.4284731
AACGCAG424050.025.3862696
TATGTAA18000.024.4514052
GTTAAGA22950.024.1011414
GTGGTTA16300.024.0997181
GGTTAAG20100.023.6717993
ACATGGG388500.023.040023
ACGCAGA471350.022.7882847
TATCAAC470600.022.761792
TCAACGC473800.022.7332124
ATCAACG468700.022.7267843
Produced by FastQC (version 0.11.5)