FastQCFastQC Report
Fri 10 Feb 2017
ERR1148369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125389
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297180.9508576372413162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165850.5306539441970263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135890.4347938768582087No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA109840.3514442522194837No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT101610.3251115301167311No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69720.22307623147070654No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG54970.17588210619542077No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA43500.13918267454067318No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42810.13697494935830387No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC41110.1315356264452201No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA39850.12750412828611096No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG35820.11460973338038882No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC35530.113681848883451No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT34420.11013029098137864No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC33410.10689869325066416No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC33130.10600280477086213No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32210.10305917119436973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG4800.033.454692
ATGGGGG60650.030.8902325
GGGTTAG13150.029.8725551
CATGGGG101700.028.7145484
TGGGGGG49800.027.7076746
ACAACGC6700.025.7433223
GTTAGAG14850.025.6327343
GGTTAGA14750.025.4028842
AACGCAG504900.024.7610346
CATGGGT39600.024.6314544
GTATCAA511150.024.4991821
TATCACG3252.3646862E-1123.792
TAACGCA4200.022.6575054
ACGCAGA559350.022.36137
CGCAGAG561900.022.2704078
TATCAAC566950.021.987762
ATCAACG567050.021.8478813
TAGAGCA17700.021.8414545
AGTACTT232900.021.80974812-13
TCAACGC575550.021.7732334
Produced by FastQC (version 0.11.5)