FastQCFastQC Report
Fri 10 Feb 2017
ERR1148368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227616
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252990.783829303114125No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA172180.5334587509790507No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT153250.47480865133894495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151580.46963455380069996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119650.3707070481742562No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG74480.23075855368172668No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC71450.22137081982491102No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70160.21737406184626673No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA68200.21130146832832652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63810.19770009815294012No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT63250.19596507143352865No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC55370.17155076688180998No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50420.15621436998701207No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG49450.1532090558480315No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC49400.15305414274808402No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT41090.12730758553681726No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA34760.10769558708346966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG94150.027.5435664
TACAACG4650.026.8600562
GGGTTAG8700.026.6851461
ATGGGGG53000.025.9232945
TATCACG2851.1823431E-1025.042462
TAACGCA4400.024.331784
TAGATCG2001.8335286E-623.7910735
GTAAGTA18550.021.8024642
CATGGGT30300.021.1999654
TGGGGGG44850.021.0857336
ACAACGC7650.020.9921233
GAGTACT207850.020.41744212-13
TATAACG3801.9463187E-1020.3469982
AGTACTT214950.020.31028412-13
ACGCATA3101.9627805E-719.186357
AGTACTC21900.019.0111315
TGTAAGT21650.018.9721031
GTTAGAG11350.018.8651683
AACGCAG632100.018.706166
ACATGGG526750.018.597013
Produced by FastQC (version 0.11.5)