FastQCFastQC Report
Fri 10 Feb 2017
ERR1148367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3905363
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252610.6468284766358466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141060.3611956174112368No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA129280.3310319680910584No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT121770.31180200150408555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114950.2943388361081928No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG59780.1530715582648783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58330.14935871518217386No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA52310.1339440149353594No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50610.12959102649356793No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG49190.1259550008539539No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC42510.10885031685914984No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41610.1065457935664367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3350.035.5063862
GGGTTAG18950.031.7265451
CATGGGG118000.029.8385434
ATGGGGG72750.029.6764225
GGTTAGA21700.026.858862
GTTAGAG23150.025.9482633
TGGGGGG61750.025.716526
TATAACG3700.024.1107522
GTATCAA504500.023.8224141
TAGAGCA24300.023.4964855
AACGCAG511800.023.439246
TAACGCA5350.023.3455144
TTAGAGC26200.022.7005634
GAGTACT195500.021.8896412-13
GTAAGTA29750.021.7901762
AGTACTT203350.021.68810312-13
ACATGGG461850.021.5958373
TCAACGC559550.021.4921654
CAACGCA560700.021.46935
ATCAACG555900.021.46213
Produced by FastQC (version 0.11.5)