FastQCFastQC Report
Fri 10 Feb 2017
ERR1148366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4635499
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340940.7354979474701645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189950.40977249698468277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158370.34164606658312296No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA135960.29330175672565134No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT126950.27386479859018414No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80520.17370298213849253No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG64770.1397260575398679No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA58000.12512137312509397No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC57780.12464677481324017No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG53920.11631973170525979No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC48750.10516667137669537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG19650.032.718371
TATCACG3650.030.9600872
ATGGGGG96950.030.4907955
CATGGGG150750.029.5518514
GGTTAGA22450.029.406822
GTTAGAG23500.028.8534093
TGGGGGG79250.028.069366
GTATCAA596200.024.9719311
AACGCAG611450.024.6201886
TTAGAGC27450.024.0514244
GTGGTTA26550.023.3184281
ACATGGG549200.022.6239343
AGTACTT256350.022.55132312-13
TAGAGCA29350.022.4944325
TCAACGC667200.022.4025064
ATCAACG661350.022.3848273
TATCAAC666600.022.3235592
ACGCAGA674400.022.2779887
CAACGCA672200.022.2712655
GTAAGTA36250.022.1496682
Produced by FastQC (version 0.11.5)