FastQCFastQC Report
Fri 10 Feb 2017
ERR1148365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4189542
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334050.7973425257462511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181100.4322668205737047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154060.3677251594565707No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA139790.33366415708447367No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT132570.3164307697595585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78870.1882544679108122No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG69100.16493449641989505No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA59120.14111327682118951No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC56340.13447770663237174No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG54980.13123152841050406No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC52780.12598035775748279No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC46470.11091904556631726No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG45020.10745804672682598No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT43970.10495180618788404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG106550.033.3781365
CATGGGG157350.032.5802844
TGGGGGG90050.031.0401136
TATCACG3550.028.4793282
GTTAAGA38350.028.3791944
GTGGTTA24950.027.915111
GGTTAAG34700.027.4223613
ACGCATA3300.027.0325167
GTATCAA596950.025.9872571
AACGCAG607750.025.6870086
CATGGGT50750.024.0232724
CGGTTAA10700.023.8997762
GGGTTAG15250.023.4211
TCAACGC668150.023.3474064
ATCAACG662600.023.3185773
TATCAAC666950.023.3088822
ACGCAGA668650.023.2763047
CAACGCA671450.023.2503765
GAGTACT238850.023.21925512-13
ACATGGG553100.023.1717033
Produced by FastQC (version 0.11.5)