FastQCFastQC Report
Fri 10 Feb 2017
ERR1148364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581892
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328220.9163313690083342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182550.5096468570241649No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA160520.4481430484224539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155720.4347423093716952No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT144960.4047023193329112No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG86670.24196709448526085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82040.2290409649425499No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC75510.21081037619224702No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA73500.2051988167147418No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG63760.17800648372424405No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG54130.15112125100365953No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT53960.15064664149561183No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC53460.14925073117782447No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC48950.13665962011138247No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC43240.12071832428225084No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT43200.12060665145682783No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38110.10639628442175252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3500.039.0852782
ACGCATA3150.033.986727
GGGTTAG9700.031.9107321
AACGCAT3700.028.9346436
CATGGGG107550.028.8681894
ATGGGGG58950.028.2509925
GTTAGAG11600.027.1754883
CATGGGT58450.026.4574684
CGCATAG4550.026.1436338
GCGTATC3051.0913936E-1125.3716491
TGGGGGG48900.023.717736
ATCACGC6850.023.4440483
ACTATAG10550.023.1145152
GGTTAGA12650.023.0387522
GGACCGT3655.456968E-1222.8130056
TAGAGCA13400.022.637315
CTATAGC10200.021.5755083
TCACGCA6900.021.5501524
GTAAGTA19600.021.5454542
AACGCAG682000.021.49716
Produced by FastQC (version 0.11.5)