FastQCFastQC Report
Fri 10 Feb 2017
ERR1148362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3934871
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303100.7702920883556283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167900.426697596947905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140410.35683507794791747No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA116520.29612152469547287No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT108290.27520597244484No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66800.16976414220440772No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG56510.14361334844268084No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA46670.11860617539939683No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC46590.11840286504945144No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG44120.11212565799488725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2550.030.3207537
ATGGGGG80400.030.0335625
CATGGGG128900.029.7145944
TGGGGGG66650.027.3952376
GTATCAA511150.026.0134161
AACGCAG523900.025.5997076
GTAAGTA31750.025.2877582
GGGTTAG13500.024.2488691
TGTAAGT33400.023.701061
TATCAAC574650.023.2034472
ATCAACG574850.023.1445273
ACGCAGA580200.023.1053737
TCAACGC582300.023.0731184
CGCAGAG584950.022.9990928
CAACGCA584500.022.9862755
AGTACTT228350.022.3089812-13
ACATGGG472600.022.1239173
GCAGAGT610300.021.9950549
GAGTACT217050.021.68924912-13
CATGGGT47800.021.6500154
Produced by FastQC (version 0.11.5)