FastQCFastQC Report
Fri 10 Feb 2017
ERR1148360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4374099
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301400.6890561919151806No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT206670.4724858765199416No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA200070.4573970547991712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169450.38739406675523347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137140.3135274258767348No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG81710.18680418527335574No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG78570.17962556403044375No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT73410.1678288488669324No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72420.1655655256088168No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC68940.15760960142877423No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA59520.13607373770003836No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC59430.13586798104020967No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC59260.13547932957164435No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC52470.11995613268012452No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC52120.11915596789190185No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50060.11444642656693413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3600.041.3015752
GGGTTAG17650.034.397921
ATGGGGG102850.031.9793595
CATGGGG153700.031.2670144
TGGGGGG85500.029.4254536
GTTAGAG21450.028.5600413
GGTTAGA20850.027.954332
GTGGTTA29100.027.4087071
GGTTAAG39300.026.7873423
GTTAAGA41750.026.7824484
ACGCATA3950.025.5976897
TAGAGCA24500.024.7618485
TTAGAGC25050.024.6930394
TACAACG5450.024.007962
CATGGGT51450.023.582714
AGTACTT226150.022.30145812-13
GTACATT17800.022.0699141
GAGTACT218800.021.73227312-13
ACAACGC7400.021.7010613
ATATGTA24050.021.5318281
Produced by FastQC (version 0.11.5)