FastQCFastQC Report
Fri 10 Feb 2017
ERR1148359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981507
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237130.7953360498566665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130760.43857015931876064No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA119390.4004350819904162No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT105940.35532366685706257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77240.25906362118217396No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG55520.18621455525678793No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC51030.17115505682193602No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA47290.15861106480715959No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG47150.15814150360874552No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44760.15012542315010496No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT42210.1415727013218483No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC39640.13295289932238966No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC32830.11011210102810423No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30670.10286744253828685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG68250.033.0280885
CATGGGG106450.032.6297724
TGGGGGG56550.029.8698226
GGGTTAG8100.029.401291
GTGGTTA20250.026.7551751
GGTTAAG25250.025.203993
GTTAAGA26500.024.6884444
TATCACG2951.8189894E-1024.1939142
AACGCAG458300.024.1384856
GTATCAA462250.023.8665641
GAGTACT166750.023.16687612-13
GTACACT34650.023.0246471
CATGGGT34300.022.8889964
ACATGGG413250.022.3946173
TACAACG5350.022.2342832
TATCAAC500350.022.0028742
ATCAACG497700.021.9766253
ACGCAGA503100.021.9653597
TCAACGC502450.021.9464264
CAACGCA504850.021.8656565
Produced by FastQC (version 0.11.5)