FastQCFastQC Report
Fri 10 Feb 2017
ERR1148355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285092
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304301.3316750485319628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179270.7845198355252218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143260.6269331825589517No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA118000.5163905873374026No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT104850.458843670189209No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73470.32151878348880486No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG58230.25482562627675387No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG47700.20874433064401784No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC45220.19789137592709613No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA42770.18716970695271787No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA42480.18590061144146494No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG38390.1680019885413804No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT37090.16231293969783275No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC37020.16200660629856478No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC35110.15364808068996783No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT33380.14607726953663136No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32510.14226998300287252No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.1415260304617932No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA31320.1370623152153174No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA27020.11824469211742895No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26100.11421859601276448No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC26020.11386850069931538No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.11321207198659836No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA23490.10279673641148802No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23330.10209654578458985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2550.044.3119747
AACGCAT2950.042.3355266
GGGTTAG7950.041.176131
TATCACG2800.038.2315752
TATAACG2300.036.1999442
GGTTAGA9850.034.4148862
ATGGGGG51800.032.3769955
GTTAGAG10250.031.9121463
CGCATAG3600.031.387658
TAGAGCA11850.031.1164845
TTAGAGC11350.029.3433274
CATGGGG87150.029.2756864
GTGGTTA12050.029.1416991
TGGGGGG44850.028.9069146
GTTAAGA16900.025.6893654
TCACGCA4450.025.392814
AACGCAG533750.025.315016
ATATGTA11000.024.8894371
GTAAGTA17050.024.765192
GTATCAA550550.024.6375481
Produced by FastQC (version 0.11.5)