FastQCFastQC Report
Fri 10 Feb 2017
ERR1148354.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148354.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767003
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209250.7562333687386678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122800.4438014703995623No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA104580.37795405353734707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98000.35417381188238684No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94990.3432956162317135No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51110.18471248495213052No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG49760.1798335599925262No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44660.1614020657006877No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC43040.15554735574916254No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG41750.150885271898874No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT39200.14166952475295475No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC34140.12338259120066007No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG33330.12045523622489747No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30920.11174545166738165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2700.044.0569462
GGGTTAG8350.032.0818181
CATGGGG82700.029.3434164
ATGGGGG48150.028.287545
ACAACGC4600.025.859983
TACAACG3450.025.8595122
GGTTAGA9850.025.3606992
ACGCATA1951.4707875E-624.401217
TGGGGGG41250.023.0702346
TCACGCA5850.022.3677774
GCGTATC2403.549685E-722.3235951
GTTAGAG10950.021.7271063
ATCACGC5750.021.7223833
TAACGCA3358.949428E-1021.3055344
AACGCAG423950.021.2546526
CTAACGC1400.003163561521.2421263
AGTACTT169350.021.21327612-13
TAGAGCA13200.021.1777575
GTATCAA424650.021.1679461
GAGTACT162700.020.70944212-13
Produced by FastQC (version 0.11.5)