FastQCFastQC Report
Fri 10 Feb 2017
ERR1148353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295587
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156870.6833546278141496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98190.4277337343346168No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88760.3866549165856053No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT82160.3579041003455761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76870.3348598855107648No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39760.1732018869247822No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39730.17307120139641843No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39320.1712851658421136No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT37040.16135306568646712No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36550.1592185353898589No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36440.15873935511919174No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC29440.12824606516764556No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG28210.12288795850473103No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC27500.11979506766678849No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC26150.11391421889041889No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25200.1097758438255662No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT24260.10568103060350142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG7700.035.562881
ATGGGGG52350.034.992575
CATGGGG80450.033.0463334
GGTTAGA8300.032.2446252
TGGGGGG43800.030.823656
GTTAGAG9350.030.5331383
TAGAGCA10600.028.0547225
GTTAAGA19300.027.7349264
AGAGCGC7800.027.4498658
TACAACG2853.6379788E-1227.1282232
GTGGTTA15400.027.0587141
GGTTAAG19150.026.3992713
AAGAGCG7550.025.2078257
CATGGGT27050.025.0656744
TTAGAGC11450.024.9331724
ACAACGC3553.6379788E-1223.4553263
TATAACG2302.3760549E-723.2722072
TATCACG1852.7013057E-522.5034542
GTACATT11250.022.2242241
TACATTG12150.022.0271912
Produced by FastQC (version 0.11.5)