FastQCFastQC Report
Fri 10 Feb 2017
ERR1148352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929823
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244540.8346579298476393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145000.494910443395386No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA131160.4476720948671643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119460.40773794184836426No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT117310.4003996145842258No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59250.2022306466977698No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG58280.19891986649022822No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50470.17226296605631125No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50460.17222883430159433No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT49440.16874739532046817No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA48650.16605098669783125No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC40760.13912103222617886No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG38890.13273839409411423No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC38360.1309294110941173No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC35490.12113359749036035No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC34410.11744736798093264No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT34380.11734497271678186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30280.10335095328284337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2450.038.8379822
GGGTTAG9950.033.50181
TATAACG2351.0913936E-1130.3679982
ATGGGGG68450.030.2359335
ACGCATA2601.8189894E-1229.7363477
CATGGGG102900.029.0138874
GTTAGAG12050.027.6387623
CATGGGT31200.027.4489354
TTAGAGC12750.026.5877914
TGGGGGG57450.026.1907586
GTTAAGA24850.025.8472884
TAACGCA4150.025.7953834
GGTTAGA12400.025.4189152
GTAAGTA22100.024.4879192
TACAACG4650.024.299842
AACGCAT3553.6379788E-1223.4540216
TAGAGCA13550.023.2623835
TGTAAGT22850.023.1849961
GTACACT39300.023.1740571
AGTACTT197250.022.41759312-13
Produced by FastQC (version 0.11.5)