FastQCFastQC Report
Fri 10 Feb 2017
ERR1148351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829999
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207950.7348059133589799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124600.4402828410893431No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA123240.4354771856809843No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT108610.38378105433959514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98440.3478446458815003No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG56600.20000007067140307No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54040.19095413107919826No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51220.18098946324716017No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT49940.17646649345105778No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC47970.1695053602492439No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44460.15710252901149435No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC41300.14593644732736655No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40320.14247354857722566No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG37770.1334629446865529No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC35460.12530039763264933No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT33430.11812725022164318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2350.040.4923252
ATGGGGG45600.026.7377555
GGGTTAG8500.026.607721
CATGGGG81650.026.150154
CATGGGT39150.024.0027644
TGGGGGG40200.022.931986
GGTTAGA9000.022.4676152
TATAACG2755.548827E-821.6265832
TACAACG3401.0768417E-920.9905052
GGTTAAG15450.020.7874493
GAGTACT166900.020.70333712-13
TAACGCA4600.020.6870274
AGTACTT174850.020.57833912-13
GTGGTTA12100.020.1670061
GTATCAA471600.019.9274311
GTACTTT213250.019.871651
GTTAGAG9650.019.7223473
TCACGCA5150.019.6325914
AACGCAG479250.019.558256
ACATGGG412050.019.4714363
Produced by FastQC (version 0.11.5)