FastQCFastQC Report
Fri 10 Feb 2017
ERR1148350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586669
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241950.9353728675760216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139020.5374479687969353No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA115010.4446258875797406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107600.41597900620450473No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT98330.38014140966625415No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55970.21637867079243614No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG54200.2095358934598899No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG46350.17918798269125272No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC45000.17396891523422595No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41310.15970346418501943No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT39150.15135295625377657No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC36570.1413787384470143No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG33030.12769318378192185No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32560.1258761751116977No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31290.12096638572619844No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30200.11675247200163609No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA27400.10592773949817313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG8000.034.2227941
ATGGGGG49450.032.113075
TATCACG2255.456968E-1231.7183362
CATGGGG83150.031.615274
GTTAGAG9400.027.8395183
TGGGGGG42700.027.5787266
GGTTAGA10000.026.167632
CATGGGT37200.025.9005474
GTTAAGA15250.025.3501424
TATAACG1901.1739885E-625.0407942
CACTATA8600.024.22241
TTAGAGC10850.024.119034
GGTTAAG15150.023.5545863
GTGTTAT9550.023.0592921
ACGCATA2603.0622687E-822.8751287
AACGCAG454200.022.7320986
TAGAGCA12050.022.7042755
GTATCAA461250.022.4651871
TTTACGC1350.00256230122.02793
ACATGGG394350.021.8687063
Produced by FastQC (version 0.11.5)