FastQCFastQC Report
Fri 10 Feb 2017
ERR1148348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16384
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.860595703125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.579833984375No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA910.555419921875No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT890.543212890625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.335693359375No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG480.29296875No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.23193359375No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC340.20751953125No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC320.1953125No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG280.1708984375No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC280.1708984375No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT270.164794921875No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT270.164794921875No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC260.15869140625No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA240.146484375No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG220.13427734375No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.128173828125No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC190.115966796875No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG180.10986328125No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA180.10986328125No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC180.10986328125No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGTA150.004159291759.64570646-47
AGAAAGC350.00767901850.9685485
GTACTTT1650.043.6464651
ACTTTTT1850.038.5707933
TACTTTT1850.038.5707932
GGGAAGC1101.9283949E-532.434537
TACATGG3100.030.6907372
CCATCTC400.005263610329.77718244-45
ATGGGAA1402.869967E-629.7316535
ACATGGG2850.029.2100433
CATGGGA1453.7725622E-628.706424
GTACATG3150.028.5780431
TGGGAAG1306.0335227E-527.4446016
CATGGGG1107.4543606E-427.0287724
GGAAGCA1300.001974518622.87058
CTTTTTT3204.0563464E-1022.2987384
AACGCAG2555.6656245E-720.987055
GAAGCAG1450.003722403720.5045879
GTATCAA2356.2043746E-620.430261
ATCAACG2801.3911376E-619.1132052
Produced by FastQC (version 0.11.5)