FastQCFastQC Report
Fri 10 Feb 2017
ERR1148345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887384
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224190.7764467767363121No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA152080.52670514209402No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT135710.4700102237873452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133980.46401864109519203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108380.37535707062171153No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG72840.252269874737825No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC63030.21829448386497952No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG63020.2182598504390133No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA61190.21192193348719812No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT58080.20115093801170886No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56540.19581739041291354No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC51050.17680363955746792No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50290.1741714991840365No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG45610.15796305583185333No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC44990.1558157834219487No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT41540.1438672514636086No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30630.10608218373448076No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA30470.10552804891902151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2700.041.8530582
GGGTTAG12550.036.9981081
ATGGGGG60350.031.438795
TTAGAGC15550.031.364324
GTTAGAG15300.031.0993273
GGTTAGA14550.030.6574252
CATGGGG95850.029.8473224
TAGAGCA15800.029.738735
TGGGGGG51300.026.2025456
TCACGCA4850.025.7531284
ATCACGC5700.023.9996763
GTGGTTA15650.023.5833781
CATGGGT36150.023.1986774
AGCGTCA4700.022.7786033
TAACGCA4200.022.658084
ATATGTA15350.022.4930461
TATGTAA14900.022.3531722
GTACTTT230000.022.1810251
GTACATT10750.022.150331
ACGCATA2454.3662476E-721.8488647
Produced by FastQC (version 0.11.5)