FastQCFastQC Report
Fri 10 Feb 2017
ERR1148344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976920
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178000.9003905064443681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110460.5587479513586792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84210.4259656435262934No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80500.40719907735264954No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT72860.3685531028063857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46020.2327863545312911No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG41170.20825324241749793No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT34180.1728952107318455No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32160.16267729599579142No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA31410.158883515772009No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31410.158883515772009No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC27920.14122979179734133No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC27670.13996519838941382No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG27560.13940877728992573No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24940.1261558383748457No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT23090.11679784715618236No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22610.11436982781296158No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20750.10496125285798111No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA20330.10283673593266293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1850.041.793682
GGGTTAG6550.031.7969511
ACGCATA1751.6785634E-830.5890087
GTGGTTA10650.029.0544341
ATGGGGG49450.028.7470025
TACAACG2701.8189894E-1228.6364082
CATGGGG75750.028.5811464
GTTAAGA15300.028.378694
TATAACG1903.7356585E-828.172662
GGTTAGA7550.027.571482
TGGGGGG41800.027.3203266
TTAGAGC8750.027.190234
GTTAGAG8100.027.1692473
TAGAGCA8650.026.8169545
GGTTAAG15150.026.304083
AACGCAT2057.802373E-826.1125686
TCACGCA3200.026.0218984
ACCGTAG1451.3438253E-424.6118453
TAACGCA3401.8189894E-1224.4911984
AGCGTCA3252.3646862E-1123.791453
Produced by FastQC (version 0.11.5)