FastQCFastQC Report
Fri 10 Feb 2017
ERR1148342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528520
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210810.8337288216031512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127350.5036543116131176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100440.397228418205116No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93100.3681995792004809No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT78120.30895543638175693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53870.21304953095091198No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG43230.1709695790422856No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36290.14352269311692215No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36060.14261307009634094No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT34950.13822315030136206No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32540.12869188299875026No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC28640.11326784047585149No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28110.11117175264581652No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG27010.10682138167781943No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC26240.10377612200022147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG11050.035.013371
ATGGGGG57000.033.806545
GTTAGAG13100.032.688263
CATGGGG88100.032.6063234
TAACGCA3850.032.440624
GGTTAGA13300.029.9602322
TGGGGGG49250.028.8617236
TATAACG3100.028.77742
TTAGAGC14450.028.3995744
TAGAGCA14850.028.0351055
TATCACG2507.057679E-1026.168252
GTGGTTA13150.024.4427951
GGTTAAG16850.022.9426753
GTATCAA404350.022.448911
GTACACT34750.022.4387841
AACGCAG406950.022.3166286
GTATTAG10800.022.0454541
AGTACTT166550.021.73084312-13
GTTAAGA19050.021.5419334
GAGTACT159150.021.45190412-13
Produced by FastQC (version 0.11.5)