FastQCFastQC Report
Fri 10 Feb 2017
ERR1148341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838707
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219950.7748245944368334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133520.47035498908481926No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA110520.3893321853928567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103290.363862843188818No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT98160.34579123523491506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54270.19117858940707863No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG51440.18120926182237196No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44180.1556342377004742No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG43720.15401378162663495No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41340.14562968280981448No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40680.14330468061691468No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC35540.1251978453570587No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC33650.11853988453193656No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30990.10916942114843131No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC29100.10251146032330917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG12050.031.1127151
ATGGGGG61000.029.1512995
GTTAGAG13600.028.424383
CATGGGG97100.027.745694
GGTTAGA13150.026.683032
TATCACG2905.456968E-1226.6596372
TTAGAGC15000.026.167924
TAACGCA3650.026.0701584
TGGGGGG51750.025.6277856
TAGAGCA15850.024.3893725
CATGGGT33650.022.4458054
GTATCAA437100.021.8785931
AACGCAG439750.021.7062956
GTATATG7500.021.4233251
GTAAGTA20600.021.363552
AGTACTC19650.020.883495
GAGTACT169100.020.68035312-13
ATATGTA14700.020.646061
AGTACTT174600.020.36953512-13
GTACTTT212100.020.2011531
Produced by FastQC (version 0.11.5)