FastQCFastQC Report
Fri 10 Feb 2017
ERR1148340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590133
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185260.7152528460893707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112750.435305831785472No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA92500.3571245183162409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87330.33716415334656563No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT84270.325350088200104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46190.17833061082191531No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG42200.16292599646427422No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT38840.149953689636787No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36820.14215486231788096No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC35450.13686555864119718No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA33180.12810152992143645No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC30980.11960775759391505No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC29670.11455010225343641No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG28160.10872028579227398No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC27560.1064038024302227No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC26310.10157779542594918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2250.034.363352
ATGGGGG61800.031.3735585
CATGGGG94400.030.6195584
GGGTTAG11450.028.599161
TACAACG3650.027.7006972
TGGGGGG51850.027.3000146
GTAAGTA22300.024.5367832
TGTAAGT22450.024.1335431
ATATGTA13350.024.0828861
TATGTAA14350.023.6242262
GTACACT37050.023.4617651
GTTAGAG14050.023.282043
GAGTACT142950.022.84184612-13
GTATCAA374850.022.649471
CGGTAAA2903.936293E-922.5594942
ACATGGG354900.022.0538583
AACGCAG385800.022.0295016
CATGGGT37650.021.9575794
ACAACGC4950.021.6272853
TTAGAGC14600.021.1828864
Produced by FastQC (version 0.11.5)