FastQCFastQC Report
Fri 10 Feb 2017
ERR1148339.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148339.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309142
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212020.6407098879407411No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA173350.5238518020683307No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT160090.48378099217259335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129950.39269998084095514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95480.2885340067002262No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT71890.21724664580728176No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG71510.21609831188870104No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG69030.2086039221042796No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC62830.18986794764322595No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA58510.17681320414778212No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC57380.17339842170568687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55660.1682006997584268No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50900.1538163064625211No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC48880.14771200510585522No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG43820.1324210324005437No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT40670.12290194860178258No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC35110.10610001021412802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3300.032.4424932
ATGGGGG68900.031.7685475
CATGGGG102050.029.783564
TGGGGGG55150.029.2271186
CATGGGT39850.025.2247664
TACAACG4250.023.791162
AGTACTC21650.022.8028415
GGGTTAG9750.022.59091
ATCACGC5350.022.2354013
ATATGTA16050.021.141521
GAGTACT167100.020.6447912-13
TATGTAA16600.020.4231342
AGTACTT174650.020.26316612-13
TATAACG2659.2829396E-720.2000432
GTAAGTA19750.020.1773152
TAACGCA3551.8426363E-920.1058144
ACATGGG498550.019.8046953
ACAACGC5200.019.4452863
GTACTTT210150.019.4042821
GTTAAGA24250.019.376894
Produced by FastQC (version 0.11.5)