FastQCFastQC Report
Fri 10 Feb 2017
ERR1148337.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148337.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2904115
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183030.6302436370460536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113010.3891374825032755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89600.30852772703560294No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA79650.27426599841948407No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70080.24131275793141801No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47840.1647317685422237No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36980.12733655519839954No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC35260.12141392472405535No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA33620.1157667654345644No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33140.1141139383254451No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32350.11139366037501959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG95800.029.6152634
ATGGGGG63000.029.5506155
ATATGTA14050.026.2636361
GTAAGTA22700.026.2016942
TGGGGGG51950.025.6454436
GGGTTAG9100.025.5071951
TGTAAGT24100.024.6957651
TATGTAA15750.023.791142
AGTACTC18600.023.024085
GTTAAGA26900.022.9955484
GTATCAA346550.022.6010951
ACATGGG321550.022.1970623
AGTACTT146550.022.16075312-13
CATGGGT27550.022.0212154
GGTTAAG25200.021.9505373
AACGCAG360750.021.5649856
GAGTACT142150.021.25663812-13
GTTAGAG12650.021.1584933
TACATGG374450.020.8242
GTACATG375250.020.8090671
Produced by FastQC (version 0.11.5)