FastQCFastQC Report
Fri 10 Feb 2017
ERR1148336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190321
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210430.9607267610546582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133730.61054977786361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97030.44299442867050076No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA75610.3452005436646044No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT67830.3096806358520053No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52180.23822992155031159No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG38060.17376448474903905No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31750.14495592198586418No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC29940.13669229304745745No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT27840.12710465726256562No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA27580.12591761664157902No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC26230.11975413649414858No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC25920.11833881883066455No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA25020.11422983206571091No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23850.10888814927127119No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22250.10158328391135364No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT22030.10057886492436496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG7500.032.5379941
CTTAGCG1501.4846046E-731.7204693
TATAACG1653.422938E-728.8361342
CATGGGG77350.028.454
ACGCATA2103.1559466E-928.3218467
AGCGTCA4050.027.9022643
ATGGGGG46700.027.6365435
CATGGGT29850.027.2971384
GTTAGAG9850.025.964093
TAGAGCA10000.025.5746295
ATATGTA11050.025.3165071
GTATCAA365800.025.0904731
TTAGAGC10750.024.8968814
AACGCAG373300.024.7112486
GGTTAGA10200.024.489512
ATGGGTG17350.024.338835
AACGCAT2451.6294507E-824.275876
TGGGGGG40150.023.7014736
TATGTAA12100.023.5931992
TACAACG2802.6229827E-923.3649922
Produced by FastQC (version 0.11.5)