FastQCFastQC Report
Fri 10 Feb 2017
ERR1148335.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148335.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088503
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104040.9558081144470894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68420.6285696961790643No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA39730.36499669729895096No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27120.2491495200288837No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19150.175929694268183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18570.1706012753295122No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG17180.15783144373511143No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT16740.1537891948850853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16040.1473583444418619No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC14810.13605842152019793No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC13840.12714710019173123No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC13720.12604466868717865No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT12160.11171305912799506No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA11040.10142369841883761No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG802.3005832E-644.6058122
GGGTTAG5850.043.7524151
GGTTAGA5900.043.345762
TAGAGCA5800.043.067685
TTAGAGC6250.042.8215754
GTTAGAG5850.042.699583
CATGGGG43550.035.5071144
ATGGGGG29050.035.0090375
AGCGTCA2550.034.984953
TATCACG1102.0456804E-532.4405862
TGGGGGG22800.031.3023246
GTGGTTA7900.030.1385481
GTACACT13350.028.9815441
GTTAAGA11600.028.7117864
AACGCAG167150.027.006336
GAGTACT66950.026.8315912-13
GTATCAA172750.025.601191
TAACGCA1401.05896026E-425.4890354
GGTTAAG11250.025.375753
ACATGGG156250.025.083933
Produced by FastQC (version 0.11.5)