FastQCFastQC Report
Fri 10 Feb 2017
ERR1148334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500178
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217810.8711779721283844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137440.5497208598747769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102100.40837092399021185No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77510.3100179267236173No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68380.27350052676249453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53640.2145447244156216No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39200.1567888366348316No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32470.12987075320237199No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32430.12971076459356093No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA29800.11919151356423421No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28290.113151943581617No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC26180.10471254446683395No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA26010.10403259287938699No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG25770.10307266122652067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2300.054.302342
ACGCATA2700.033.0417637
ATCACGC3600.031.3903053
TCACGCA4400.027.0347144
CATGGGG61350.026.369314
AACGCAT3400.026.2390486
GTATCAA393350.024.8990691
CGTATAG1200.001283464624.807621
AACGCAG397950.024.7196756
CATGGGT38600.024.653424
ATGGGGG30550.024.5303525
GAGTACT172300.023.2831212-13
ATCAACG433900.022.5897793
AGTACTT179150.022.57545512-13
TCAACGC440100.022.5688574
CAACGCA443000.022.4345425
ACGCAGA440650.022.3242827
TATCAAC440500.022.3044362
CGCAGAG442950.022.2352188
GCAGAGT457100.021.5729269
Produced by FastQC (version 0.11.5)