FastQCFastQC Report
Fri 10 Feb 2017
ERR1148332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484543
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151740.6107360589049978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97200.39121882776832595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74280.2989684622081405No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60450.24330430183740026No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53500.21533135067495307No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37790.15210040639264444No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC30440.12251750120645931No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG28280.11382374947827428No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT26420.10633746326789273No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24980.10054162878243605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1902.910383E-1134.4322742
ATGGGGG62350.032.622435
CATGGGG90100.031.8822484
GCGTATC1352.3264147E-630.8613131
TATCACG1802.2100721E-829.7369652
TGGGGGG51000.029.387126
GGGTTAG11400.027.6707631
TAACGCA3050.027.2995074
GTTAAGA21300.026.8051514
GGTTAAG19750.024.6929723
GGTTAGA14000.024.6391982
GTATCAA282700.024.5694351
GTGGTTA15050.024.5191441
GTAAGTA21650.024.4488682
ACAACGC3950.024.0907063
ATATGTA13150.023.9883441
GTTAGAG13900.023.5328483
TACAACG3302.910383E-1123.4291232
AACGCAG296150.023.4160396
TGTAAGT22700.022.8109571
Produced by FastQC (version 0.11.5)