FastQCFastQC Report
Fri 10 Feb 2017
ERR1148331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161548
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204530.6469299216712825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125390.39660950901267356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95410.30178254450035236No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA90040.2847971942858372No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT79100.25019389235905953No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51220.16200924357308508No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40710.12876603486646415No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37830.11965657329890295No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37130.11744246805678736No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36060.11405805004383927No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC35960.11374174929496562No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT35760.11310914779721831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2550.034.9845432
ATGGGGG70600.031.6768955
CATGGGG107350.029.9192854
TGGGGGG59100.027.8773696
GGGTTAG11850.025.6149221
TATGTAA17600.025.0059992
CATGGGT35150.024.3666954
GTATCAA370850.024.0732211
ATATGTA18400.023.289271
AACGCAG386800.023.25016
TACCGTA1552.1125827E-423.0239057
AGTACTC19000.022.8522385
ACATGGG351750.022.607723
GTAAGTA24050.022.0089872
GAGTACT153200.021.85672212-13
CAACGCA415450.021.7183235
TCAACGC415400.021.706624
ATCAACG410400.021.6667273
AGTACTT159450.021.50355112-13
GGTTAGA13650.021.3495432
Produced by FastQC (version 0.11.5)