FastQCFastQC Report
Fri 10 Feb 2017
ERR1148330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3378404
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234790.6949731293237872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138970.4113480803361587No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA132990.3936474145780079No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT117350.3473533656720747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113960.3373190417723872No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60050.17774665196939146No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG59950.17745065421423842No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG56340.16676513525321424No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC56200.166350738396No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA54190.16040118351742422No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52270.15471802661848613No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC43910.12997261428769324No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC43390.12843342596089752No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG40500.1198790908369751No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC39140.11585352136689396No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT34990.10356961452804342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG72750.026.0786485
GGGTTAG10350.025.3023721
CATGGGG110100.025.2264964
GTGGTTA19950.023.5685621
GGTTAAG25100.023.2209383
TGGGGGG60850.022.9686096
GTTAAGA27950.022.5554494
GTTAGAG12950.022.5036893
GAGTACT178550.022.25071712-13
AGTACTC23500.021.2588045
AGTACTT185250.021.07676312-13
GTAAGTA25550.020.7166752
CATGGGT35600.020.5486134
GGTTAGA12400.020.144132
GTATCAA505900.020.0472051
TACAACG4453.6379788E-1220.047132
TTAGAGC16450.019.8849354
GTACTTT225500.019.874551
AACGCAG518050.019.757716
CTATACG1856.979606E-419.2888764
Produced by FastQC (version 0.11.5)