FastQCFastQC Report
Fri 10 Feb 2017
ERR1148328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803651
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194450.6935599331015165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121940.43493287859294893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94700.3377738527370204No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64830.23123420140381237No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58160.20744379382455236No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49650.17709051518894472No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30020.10707466799541028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2200.035.1453867
TATCACG2255.456968E-1231.721532
ATGGGGG54200.027.4339525
CATGGGG85850.026.8806064
AACGCAT3051.0913936E-1125.350776
GTATCAA309100.025.3266261
TATAACG3451.8189894E-1224.1359482
TGGGGGG45850.024.1279956
AACGCAG325300.023.9881866
GGGTTAG9750.023.8128051
TAACGCA4050.023.497014
GTAAGTA19050.023.1042632
ATCAACG349250.022.3602143
TCAACGC351850.022.2625984
CAACGCA355300.022.0799065
TATCAAC357000.021.9751572
ACATGGG287550.021.8216083
AGTACTT154950.021.80201512-13
GGTTAGA10950.021.7270762
GAGTACT149600.021.58778812-13
Produced by FastQC (version 0.11.5)