FastQCFastQC Report
Fri 10 Feb 2017
ERR1148327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6479495
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT579010.8936035910205965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272070.4198938343188782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264140.4076552262174753No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT159960.24687109103410065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130380.20121938515270094No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA129960.20057118648907055No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87670.13530375438209305No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG80360.1240220109746207No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA74830.11548739523682015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA924750.028.6290741
AACGCAG922550.028.4633356
TATCAAC1029550.025.6308822
ACGCAGA1038550.025.1982467
ATCAACG1045650.025.1807253
TCAACGC1056850.025.0320514
ATATGTA21900.025.0040651
CGCAGAG1046400.024.9751078
CAACGCA1065800.024.760465
GCAGAGT1084000.024.1197859
TATGTAA22950.023.8411222
AGTACTT435900.023.28436912-13
ACGCATA5500.022.7091887
GAGTACT421550.021.88304112-13
CATGGGG190950.021.7099174
ATGGGGG103450.021.4448665
ATGTAAG27850.020.9288773
GTAAGTA32500.019.9464072
CGTATCA7200.019.8244112
AACGCAT6650.019.6764186
Produced by FastQC (version 0.11.5)