FastQCFastQC Report
Fri 10 Feb 2017
ERR1148326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461330
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146710.5960598538188703No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA111990.4549979076353029No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT105080.426923655097041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96710.3929176502135025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72280.2936623695319197No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT48390.196601024649275No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG48130.19554468519052706No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG47740.1939601760024052No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC45630.18538757501025868No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44990.18278735480410999No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC36650.1489032352427346No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC35790.1454091893407223No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35200.14301211133817895No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC34490.14012749204698272No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG32370.13151426261411514No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT29260.11887881754986125No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG45300.030.1960535
CATGGGG71800.028.9082324
TATAACG2803.6379788E-1227.6119462
ACGCATA1755.727452E-727.18777
TGGGGGG36650.025.1523326
GGGTTAG7700.023.9626981
TAACGCA3205.0567905E-1022.302414
AGCGTCA5550.021.4317463
GAGTACT126700.021.26472312-13
GTTAAGA16400.021.0331674
AGTACTC16650.020.7173545
AGTACTT133000.020.68227212-13
GGTTAAG14400.019.8243643
CGGTTAA4203.45608E-1119.8239612
CATGGGT21200.019.356814
ACATGGG342400.018.7416653
GTTAGAG11000.018.3825913
ACCGTCG5900.018.1443358
GGTTAGA10050.017.7528022
TACCGTC7050.017.715397
Produced by FastQC (version 0.11.5)