FastQCFastQC Report
Fri 10 Feb 2017
ERR1148325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3514550
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219860.6255708412172255No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA141630.40298188957334513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134190.3818127498541776No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT121920.3469007412044216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106270.302371569617732No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC61530.17507219985488898No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT58100.16531277119403623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57850.16460144257444054No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG57810.16448762999530525No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG57020.16223983155738286No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA56930.16198375325432843No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC46690.13284773299568933No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC46640.1327054672717702No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC43310.12323057005875575No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC41130.1170277844958814No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG37090.1055327140032152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG17000.035.365561
ATGGGGG87200.033.9669235
CATGGGG128450.032.3195044
GTTAGAG19100.031.7621693
GGTTAGA18700.031.4860672
TGGGGGG73600.029.414876
GTGGTTA23750.028.8232061
GTTAAGA35600.028.5686324
GGTTAAG32350.028.3132533
TTAGAGC22150.026.583054
GCGTATC2057.7458026E-826.1334481
TAGAGCA23700.026.099275
ATATGTA19200.025.112611
TATGTAA20150.025.0881542
ACGCATA2651.3878889E-924.6882427
TAACGCA3750.023.7904874
GTAAGTA32200.022.3488062
GCGGTTA11250.022.2231081
CATGGGT36400.022.2218864
TGGGGGC32000.021.7459936
Produced by FastQC (version 0.11.5)