FastQCFastQC Report
Fri 10 Feb 2017
ERR1148324.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148324.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384110
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171090.7176262840221299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107540.45106979124285373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76370.3203291794422237No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA56610.2374470976590845No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48640.20401743208157344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43510.18249996854171996No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26650.11178175503647064No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24480.10267982601473925No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24340.10209260478753078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2250.042.293352
AGCGTCA5100.032.6556933
ATGGGGG53650.031.707895
CATGGGG80900.030.659044
TATAACG1653.4229015E-728.8363722
TGGGGGG46800.026.3084686
GTGTACG2057.756171E-826.1290681
GTATCAA291450.025.6484361
AACGCAG296050.025.395286
TCACGCA4000.025.2790054
GCGTATC1951.4624347E-624.4169081
TCAACGC325650.023.0504464
ATCAACG321650.023.022733
GTGGTTA15550.022.9644551
ATAGGCG1300.00205681722.8769263
CAACGCA328200.022.8532285
ACATGGG276700.022.8289763
TATCAAC327400.022.7799872
GTAAGTA17550.022.7055642
ACGCAGA333900.022.4452887
Produced by FastQC (version 0.11.5)