FastQCFastQC Report
Fri 10 Feb 2017
ERR1148323.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148323.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253351
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189260.8399046575522411No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA126420.5610311043419334No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT122790.5449217631873596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109140.4843453150441276No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62100.2755895552889896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59610.26453934606725715No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG52910.2348058513742422No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC52040.23094493489917906No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA48950.2172320246601617No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC45260.2008564134038594No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40980.18186247948056028No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT32930.1461379074986542No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32500.14422963843626668No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31090.13797229104564712No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30740.13641904878556427No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG29270.12989543129321618No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA24610.10921512005896995No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA23090.10246961081518148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG69350.028.9026284
ATGGGGG43050.027.770065
GTACACT22100.026.3946271
TATAACG2757.6397555E-1125.9521642
TGGGGGG35650.024.1914756
CATGGGT28850.023.9147644
GAGTACT142050.023.469712-13
GGGTTAG8150.022.640491
TAACGCA3705.456968E-1222.5050644
GTACATT8700.021.893361
AGTACTT147900.021.51586312-13
AACGCAG431250.020.9637226
GTATAGG4001.6370905E-1120.8329011
TGTAGCT37850.020.58547
GTTAAGA17650.020.556024
GTACGTT1450.0038600220.5250261
GTATCAA447250.020.3488031
GTGGTTA13400.019.9889241
GGTTAGA9250.019.9317282
TTAGAGC10450.019.920754
Produced by FastQC (version 0.11.5)