FastQCFastQC Report
Fri 10 Feb 2017
ERR1148322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2063675
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147430.7144051267762608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95620.46334815317334366No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA92930.45031315492991875No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT83860.4063624359455825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62230.30154942033023613No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39640.1920845094309908No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG39370.19077616388239427No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT38970.18883787418076975No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35650.17275006965728615No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC34360.16649908536954705No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA31410.15220419882006614No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC30720.14886064908476382No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28740.1392661150617224No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25680.12443819884429476No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG25140.12182150774710165No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT24770.12002858977309895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG41250.033.0179185
CATGGGG65800.030.6416384
ACGCATA2151.2187229E-1030.4293277
AACGCAT2351.0913936E-1130.370476
GGGTTAG8800.028.4053081
TGGGGGG33850.027.7610936
TATCACG1505.313046E-627.7559092
TTAACGC2256.5847416E-926.4335584
GGTTAGA9700.025.7529032
TAGAGCA9800.025.48955
GTTAGAG10350.024.1349873
TTAGAGC10600.024.1268544
GAGTACT117500.022.27221712-13
CATGGGT27900.021.3173854
TAACGCA3451.2914825E-920.6871325
ATATGTA10700.020.5802571
TATGTAA10750.020.4711342
GTACTTT148850.020.3917351
GGTTAAG12400.020.1449283
ATGGGTG15150.020.0214585
Produced by FastQC (version 0.11.5)