FastQCFastQC Report
Fri 10 Feb 2017
ERR1148319.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148319.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297228
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153780.6694154868389207No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA142240.6191810303548451No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT126950.5526225520496877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96160.41859145021739247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72540.31577187810700547No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT63990.27855310835493907No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62630.27263292977449344No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG61050.265755075247211No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC58050.2526958577903456No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA53000.23071284173795548No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC47340.20607445146933612No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC45180.19667181490039298No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41420.180304262354455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39710.17286050840404174No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC38680.16837684374385128No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT36750.1599754138466012No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA27910.1214942530737045No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25760.11213514722961762No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG25540.11117747128278081No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC24820.10804325909313311No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC23180.10090422021671336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2250.039.6499562
TATCACG1852.8838258E-828.9337542
AGTACTC18250.027.701265
TAACGCA3450.027.583184
CATGGGG62800.027.1810174
ATGGGGG34750.025.3308835
TTAACGC2851.1823431E-1025.0426244
TAGATCG1701.4038926E-524.4902135
CATGGGT23700.023.8406834
AAGTACT23700.023.8406834
ATTAACG3052.764864E-1023.4004823
AAAGTAC23350.021.9055943
TGGGGGG29750.020.7916936
ATGGGTA12900.020.7475225
GAGTACT136550.020.53593812-13
GTGGTTA11200.020.1935021
GTATAGA5700.019.839231
GTACTTT168650.019.833351
GTTAAGA14550.019.6210254
AGTACTT141150.019.15038512-13
Produced by FastQC (version 0.11.5)