FastQCFastQC Report
Fri 10 Feb 2017
ERR1148317.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148317.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089277
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182460.8733164630635383No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA124620.5964742827303416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114190.5465527069890684No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT106760.5109901654974425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89000.4259846827395314No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG54990.26320109779603185No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51820.24802838493890472No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC49490.23687620167167878No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT48830.23371721413675642No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46410.22213425984204102No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA42650.2041376035824833No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41600.1991119415951068No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC38350.18355632115798912No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG36850.17637680403316552No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC34600.1656075283459302No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT33890.1622092235735137No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA22490.10764489342485462No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21980.1052038576024146No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21280.10185341627749694No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG21180.10137478180250871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2300.038.7874262
ACGCATA2057.8051016E-826.111817
AGTACTC16450.025.6708855
CATGGGT33000.025.2326264
CATGGGG45700.022.3851814
TAACGCA3205.0567905E-1022.303844
AAGTACT21100.022.2686044
AACGCAT2255.095848E-621.1473456
AGTACTT155200.021.0775112-13
GAGTACT149850.020.67899112-13
TCACGCA4650.020.4651724
GCTTGTA9050.020.3889181
TGAACCG2055.9577098E-520.3091875
ATCACGC4403.6379788E-1220.2762183
ATGGGTG18200.020.2613645
AAAGTAC22450.019.8697913
GTACTTT187550.019.6451191
GTATACG1550.005677282319.2008021
ATGGGTA13800.018.532665
AACGCAG448000.018.427226
Produced by FastQC (version 0.11.5)