FastQCFastQC Report
Fri 10 Feb 2017
ERR1148316.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148316.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2296169
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155700.678085977121022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102740.44744093313689015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81260.35389381182308444No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80000.3484064108521629No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69980.30476850789292953No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40390.17590168667898573No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37900.16505753714121216No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT37050.16135571902590795No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC34840.15173099192611694No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33410.14550322733213453No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA31450.13696727026625655No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28680.1249036982905004No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC25770.11223041509575296No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG25010.10892055419265742No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24350.10604620130312709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG48550.032.0985035
CATGGGG75400.029.7401184
TGGGGGG41800.026.3249866
GGGTTAG8400.026.2178961
GTTAAGA18450.024.823734
GGTTAAG18200.024.5110863
GTAAGTA19700.023.24712
ATATGTA12250.022.8369051
TAGAGCA11500.022.7576565
CTATAGC5750.022.7576563
CATGGGT24350.022.4730264
GTACATT10650.022.3555741
TATAACG1602.6193995E-422.3036332
AGTACTC15250.021.8419255
GAGTACT123050.021.55933612-13
TATGTAA13100.021.3388422
TGTAAGT21250.021.2877671
TACAACG3651.1459633E-1021.183362
GCGGTTA5950.021.0076641
GTGGTTA15400.020.871251
Produced by FastQC (version 0.11.5)