FastQCFastQC Report
Fri 10 Feb 2017
ERR1148315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729514
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148310.8575241368384414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96420.5574976554107107No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73080.42254644946499426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72060.4166488389223793No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60340.34888413739351054No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36810.21283435693495398No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33100.19138324407897248No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30530.1765235783000311No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG29570.1709728860246289No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24960.1443179991604578No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23510.13593414103615234No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC22760.13159766269599438No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21660.12523749446376264No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21530.12448583821813528No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA21080.12188395121404047No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19700.11390483106814978No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT19130.11060910752962973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1601.70985E-1037.1724052
TACAACG1555.144102E-934.534362
CATGGGG52150.026.9160214
ATGGGGG30250.025.9538465
GGGTTAG5050.024.7497041
GTACTTT166500.023.9140951
ACGCATA1751.7565355E-523.7910277
TGGGGGG25500.023.3245336
TACTTTT178850.022.3803422
GTTAGAG6450.022.1311873
AACGCAG305400.021.5591556
GTATCAA307650.021.3770331
TCACGCA3358.949428E-1021.3053954
CATGGGT19000.021.2867074
AGTACTC13800.021.1188455
TATGTAA7400.020.896922
ACTTTTT194900.020.6294693
GAGTACT126900.020.62204212-13
GGTTAGA6200.020.1450422
ATCACGC3251.4621037E-820.1308693
Produced by FastQC (version 0.11.5)