FastQCFastQC Report
Fri 10 Feb 2017
ERR1148313.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148313.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3469821
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429541.237931293862133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240930.6943585850682211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205600.5925377706803896No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA140330.40443008443375034No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT127010.3660419370336395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100390.28932328209437896No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG79250.2283979490584673No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG55210.15911483618319217No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA50230.14476251080387142No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG49710.1432638744188821No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC46210.13317689875068484No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46040.13268695993251525No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT42770.12326284266537092No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC42690.12303228322152641No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA42510.12251352447287626No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC39810.11473214324312407No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA37440.10790181971923048No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT37270.1074118809010609No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.10187845424879266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3800.042.2606772
CGCATAG5100.035.0168271
ATGGGGG61450.032.1345675
CATGGGG111550.031.9384274
AACGCAG632350.027.6438376
GTATCAA644000.026.9265231
CATGGGT48550.025.23684
ACGCAGA707200.024.5834527
CGCAGAG709550.024.5104148
TGGGGGG58100.024.3644756
TATCAAC718350.024.0942362
ATCAACG718550.023.9716433
TCAACGC730200.023.7358074
GCAGAGT732900.023.6399089
CAACGCA734400.023.6324585
GGGTTAG12350.023.6186371
ATAGTAC8600.023.5145534
TCACGCA7350.023.4675064
AGTACTT301900.022.96309712-13
TACAACG5700.022.9564172
Produced by FastQC (version 0.11.5)