FastQCFastQC Report
Fri 10 Feb 2017
ERR1148310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156109
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417571.0047137839743858No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA242880.5843927577452853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225620.5428635293251451No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT217240.5227004392810679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185050.4452481876678403No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG112740.27126333789609464No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG93560.22511440388113013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92030.22143307598525447No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT85270.20516786253681027No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC77800.18719432045694662No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG67830.16320553671715538No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC64290.15468795452669792No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC63030.1516562727300944No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT54600.1313728778528186No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA51590.12413052689426576No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA51300.12343275886171418No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC49560.11924615066640457No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42930.10329372978427659No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA41620.10014174315447454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG4750.035.061272
TATCACG4450.029.4053172
CATGGGG118650.028.9756874
ATGGGGG59500.028.3906295
CGCATAG6550.027.2685071
GGGTTAG10050.026.0656191
TAACGCA6750.024.673344
TATAGCG1552.1122694E-423.024665
TGGGGGG53650.022.5060866
TACAACG5550.022.5055452
CATGGGT42950.022.434974
AACGCAG807550.022.037686
AGTACTT305400.021.53173612-13
GTATCAA828400.021.2875841
GAGTACT294500.021.27838112-13
GGTTAGA11150.020.8042932
ATATGTA15450.020.4234351
ACGCAGA880000.020.2233267
GTTAGAG11500.020.1716043
CGCAGAG884850.020.112488
Produced by FastQC (version 0.11.5)